Study on Protective Effects of Scutellaria amoena Ethanol Extract and Its Different Solvent Parts on CCl4-induced Liver Injury of Mice / 中国药房

ABSTRACT

OBJECTIVE：To study the protective effects of Scutellaria amoena enthanol extract and its different solvent parts on liver injury induced by CCl 4. METHODS ：S. amoena was extracted with 95％ ethanol to obtain ethanol extract ，and then was respectively extracted with ethyl acetate and n-butanol to obtain corresponding polar parts. Totally 48 mice were randomly divided into normal group （8 mice）and modeling group （40 mice）. Normal group was given constant volume of olive oil intraperitoneally ， 3 times a day ，for consecutive 6 weeks. Model group was given 30％CCl4-olive oil solution intraperitoneally to induce liver injury model，with initial dose of 5 mL/kg after each 3 mL/kg，3 times a days ，for 6 consecutive weeks. After modeling ，the mice were randomly divided into model group （normal saline ），sylibin group （positive control ，20 mg/kg），S. amoena ethanol extract group （100 mg/kg），S. amoena ethyl acetate group （100 mg/kg），and S. amoena n-butanol group （100 mg/kg），with 8 mice in each group. After they were given relevant medicine intragastrically once a day ，for consecutive 6 weeks. The general information during experiment of mice was observed. 1 h after last medication ，the serum contents of TC ，TG，ALT and AST were determined by Enzyme-labelled meter . After HE staining ，the pathological changes of liver tissue were observed and Ishak score was performed. RESULTS：In normal group ，mice had normal activity ，thick and glossy hair ，and the body weight was increased. The liver tissue had no obvious pathological changes. The model group had sparse hair ，and they were emaciated and listlessness ；and body weight （before medication ，1，2 week after medication ）was significantly lower than normal group （P＜0.05 or P＜0.01）. Compared with normal g roup，the contents of TC ，TG，ALT and AST in serum were increased significantly （P＜0.01 or P＜0.05）. The structure of hepatic lobule was severely damaged and had more inflammatory cell infiltration ；the arrangement of hepatic cord FF117（-022）] was disordered and the Ishak score was significantly increased qq.com （P＜0.001）. Compared with model group ，above symptom and liver injury of mice in different administration groups wer improved to different extents. The serum contents of TC ，ALT and AST in silybin group and S. amoena ethyl acetate group ，serum contents of TG in administration groups as well as Ishak scores of liver tissue were decreased significantly in silybin group ，S. amoena ethanol extract group and S. amoena ethyl acetate group （P＜0.05 or P＜0.001）. CONCLUSIONS ：S. amoena ethanol extract and its different solvent parts can protect liver tissue of CCl4-induced liver injury model mice ，and active part is the ethyl acetate part of S. amoena .