Re-constitution of T lymphocyte subsets in bone marrow of patients with hematological malignancies after allogenic hematopoietic stem cell transplantation / 第二军医大学学报

ABSTRACT

Objective: To explore the re-constitution rule of T lymphocyte subsets in bone marrow of patients with hematological malignancies after allogenic hematopoietic stem cell transplantation (allo-HSCT), and its differences with those in the peripheral blood. Methods: This study was a prospective study. We collected the bone marrow and peripheral blood samples from 41 patients with hematological malignancies receiving allo-HSCT treatment in Department of Hematology of our hospital from Sep. 2015 to Jan. 2017. During the same period, bone marrow and peripheral blood samples of 7 healthy donors were collected as control samples. Flow cytometry was used to evaluate the distribution of T lymphocyte subsets, including CD4+ T cells, CD8+ T cells, T-helper cell (Th)1 and h2 before transplantation and 15, 30, 60, 90 and 180 d after transplantation. Luminex technique was used to evaluate Th1-related cytokines (interleukin 2 receptor [IL-2R] and interleukin 18 [IL-18]). Results: The proportions of CD4+ T cells in bone marrow of the patients with hematological malignancies were significantly lower versus the healthy controls 15 and 30 d after transplantation (both P 0.05). The ratio of CD4/CD8 T lymphocytes in bone marrow was gradually decreased after transplantation, and significantly lower versus the healthy controls 180 d after transplantation (P = 0.040). The ratios of Th1/Th2 in bone marrow were significantly higher than those of the healthy controls 15, 30, 60 and 90 d after transplantation (all P 0.05). The levels of IL-2R in bone marrow and peripheral blood of the patients with hematological malignancies were significantly higher than those of healthy controls 15, 30, 60 and 90 d after allo-HSCT (all P 0.05). Conclusion: After allo-HSCT, the re-constitution rules of different T lymphocyte subsets in bone marrow of patients with hematological malignancies are different, and are different from those in peripheral blood.