Expression of musashi-1 in hepatocellular carcinoma and its relationship with clinical prognosis / 第二军医大学学报

ABSTRACT

Objective To study the expression of Musashi-1 in tumor tissues and adjacent non-tumor tissues of patients with hepatocellular carcinoma (HCC), and to explore the relationship between Musashi-1 and clinical prognosis and the effect of Musashi-1 on the metastasis and invasionof HCC cells. Methods We collected HCC tissues and adjacent non-tumor tissues from 138 patients undergoing radical resection of HCC in our hospital from June 2000 to June 2010. Theexpression of Musashi-1 in all tissues was detected by immune histochemical method. The relationship between Musashi-1 expression and clinical characteristics and prognosis was analyzed by Pearson X test and Fisher exact test The expression of CD133 in HCC tissues and adjacent non-tumor tissues was detected by qPCR and the correlation between Musashi-1 and CE133 in tumor tissues was analyzed. Finally, we constructed SMMC-7721 cells with stable over expression of Musashi-1,determined the effect of Musashi-1 on the sphere-forming potential of SMMC-7721 cells using Sphere formation assay, and detected the effect of Musashi-1 on the ability of cell invasion by tumor invasion experiment Results Musashi-1 was highly expressed in 105 (76. 1%) HCC tissuescompared with the adjacent non-tumor tissues (P0. 05). Spearman analysis indicated that HCC patients with higherMusashi-1 expression have significantly worse overall survival rate (P<0. 05) and disease-free survival (P<0. 05) compared with those with low Musashi-1 expression. The expression of CD133mRNA in HCC tissues was significantly higher than that in the adjacent non-tumor tissues (P〈0. 05),and CD133 expression was positively correlated with Musashi-1 expression (R2 =0. 78, P<0. 001). In vitro experiments confirmed that overexpression of Musashi-1 enhanced the sphere-forming potential and invasive ability of SMMC-7721 cells. Conclusion Musashi-1 has higher expression in HCC tissues and may promote HCC progression and metastasis. High level of Musashi-1 may indicate poor prognosis,providing a reference for prognosis judgment in HCC patients.