Preparation and characterization of thermo-sensitive liposomes loaded with IR-780 and doxorubicin / 第二军医大学学报

ABSTRACT

Objective To prepare and characterize a thermo-sensitive liposomes co-loaded with IR-780 and doxorubicin (D0X). Methods Membrane hydration method and ammonium sulfate gradient method were used to prepare IR-780/D0X thermo-sensitive liposomes (DITSL). The particle size, zeta potential and polydispersity coefficient (PDI) of liposomes were measured by Malvern laser particle size analyzer, and the drug release characteristic of DITSL induced by laser was also detected. Results DITSL loading both IR-780 and D0X was successfully prepared. The encapsulation efficiency of IR-780 and D0X was (94.47 ± 8.57) % and (92.52 ± 7.61)%, respectively; the average particle size was (138.98 ± 8.74) nm, with slight negative potential; the PDI was 0.32 ± 0.02. The drug release rate of DITSL was about 80.1% after laser radiation (0. 8 W/cm2, 5 min) and the highest temperature of DITSL was 54.2°C. Conclusion The prepared DITSL has high drug encapsulation efficiency, appropriate particle size, high photo-thermal conversion efficiency, good temperature sensitivity and laser-induced thermal drug release property, which lays a foundation study for the combination treatment of tumors with photo-thermal therapy and chemotherapy.