Research on antibacterial components of chloroform and ethyl acetate extracts of artemisia anomala / 第二军医大学学报

ABSTRACT

Objective To study the antibacterial components of chloroform and ethyl acetate extracts of the traditional Chinese medicine Artemísia anómala S. Moore. Methods The chloroform and ethyl acetate extracts of Artemísia anómala S. Moore was isolated by silica gel column chromatography. The antibiotic tests of the different isolated fractions in vilro were performed by using Kriby-Bauer method and broth dilution method. The compound structures of the significantly active components were identified by the method of chemical colored-reaction and spectrum analysis. Results Multiple components of chloroform and ethyl acetate extracts of Artemísia anómala had different degrees of antibacterial activities for common pathogenic strains in clinic. The structures of significantly active components were identified as apigenin, eupatilin, and caffeic acid; eupatilin had the strongest antimicrobial activity for Standard Slaphylococcus aureus (ATCC25923) and Standard Staphylococcus aureua (ATCC29213), with the minimal bactericidal concentrations being both 0. 062 5 mg/mL. Conelusion The proposcd method in the present study is simple and quick; it can aceurately and cffectively obtain the antibacterial components of the chloroform and ethyl acetate extracts of Artemísia anómala. This study is the first to report the antibacterial activity of eupatilin.