MiR-141 inhibits the proliferation of head and neck squamous cell carcinoma / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 1314-1318, 2015.
Artículo
en Chino
| WPRIM
| ID: wpr-838815
ABSTRACT
Objective To study the effect of miR-141 on the proliferation of head and neck squamous cell carcinoma (HNSCC) cells. Methods The expressions of miR-141 in 19 HNSCC tissues and the matched adjacent normal tissues were analyzed by qRT-PCR. The miR-141 in Hep-2 or SCC-9 cells was overexpressed or down-regulated by miR-141 mimics or miR- 141 ASO transfection. Then the effects of overexpression or down-regulation of miR-141 on cellular proliferation were analyzed by MTT experiment. The targeted genes of miR-141 were predicted by bioinformatics algorithms. Results The expression of miR-141 in HNSCC tissues was found significantly lower than that in matched normal tissue (P<0. 05). Overexpression of miR141 significantly inhibited the proliferation of Hep-2 and SCC-9 cells, and down-regulation of miR-141 significantly promoted the proliferation of Hep-2 and SCC-9 cells (P<0. 05). Bioinformatics algorithms showed that ZEB1 was likely to be a targeted gene of miR-141 in HNSCC. Conclusion miR-141 may inhibit HNSCC cell proliferation via ZEB1.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Academic Journal of Second Military Medical University
Año:
2015
Tipo del documento:
Artículo
Similares
MEDLINE
...
LILACS
LIS