Relationship between ApoB gene Msp I/Xba I/EcoR I polymorphisms and serum lipid level in male Han population in Chongqing, China / 第二军医大学学报

ABSTRACT

Objective To explore the relationship between apolipoprotein B (ApoB) gene MspI/XbaI/EcoRI polymorphisms and the levels of serum lipid in male Han population in Chongqing. Methods The ApoB gene MspI/XbaI/EcoRIpolymorphisms were detected by gene chip technology in 157 dyslipidemia cases and 180 healthy controls; and their relationship with serum lipids was analyzed in the dyslipidemia group and the controls. Results In both dyslipidemia group and control group, the total cholesterol (TC) levels with M+M- genotype at MspI locus were significantly higher than those with M+M+ genotype (dyslipidemia group [6.54±0.58] vs [5.58±0.83] mmol/L, P<0.01; control group [5.43±0.17] vs [4.39±0.62] mmol/L, P<0.01); the high density lipoprotein cholesterol (HDL-C) levels with X+X- genotype at XbaI locus were significantly lower than those with X-X- genotype, whereas the low density lipoprotein cholesterol (LDL-C) levels were significantly higher than those with X-X- genotype (dyslipidemia group HDL-C [1.08±0.27] vs [1.22±0.44] mmol/L, P=0.03; LDL-C [3.88±0.63] vs [3.46±0.83] mmol/L, P=0.01; control group HDL-C [1.31±0.43] vs [1.48±0.37] mmol/L, P=0.04; LDL-C [3.19±0.54] vs [2.94±0.59] mmol/L,P=0.02); the HDL-C levels with E+E- genotype at EcoRI locus were significantly lower than those with E+E+ genotype (dyslipidemia group [1.01±0.18] vs [1.21±0.43] mmol/L, P=0.01; control group [1.27±0.20] vs [1.47±0.40] mmol/L, P=0.03). The HDL-C levels in dyslipidemia group with X+X- genotype were significantly lower than the control group with X+X- genotype, but the LDL-C levels were significantly higher than the control group with X+X- genotype (HDL-C [1.08±0.27] vs [1.31±0.43] mmol/L, P=0.01; LDL-C [3.88±0.63] vs [3.19±0.54] mmol/L,P<0.01); the HDL-C levels in the dyslipidemia group with E+E- genotype were significantly lower than those in the control group ([1.01±0.18] vs [1.27±0.20] mmol/L, P<0.01). The multiple linear regression analysis showed that ApoB gene MspI polymorphism was positively correlated with TC; XbaIpolymorphism was negatively correlated with HDL-C and positively correlated with LDL-C. Conclusion The ApoBgene MspI and XbaI polymorphisms may be associated with the levels of serum lipids. There is a tendency that the M+M- genotype can increase TC levels and the X+X- genotype can reduce HDL-C levels and increase LDL-C levels.