Real-time fluorescent quantitative reverse transcription polymerαse chain reaction in detecting expression of ERα, PR and Her2 mRNA in breast cancer tissues / 第二军医大学学报

ABSTRACT

Objective To evaluate the clinical value of real-time fluorescent quantitative reverse transcription polym ERαse chain reaction (qRT-PCR) in detecting expression of estrogen receptor alpha (.ERα), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HerZ) genes in breast cancer tissues. Methods Totally 48 breast cancer tissues and 28 benign breast tumor tissues (control) were obtained from patients undergoing surgery in our hospital during Mar. 2010 and Oct. 2010. The expression of ERα, PR and Her2 protein was examined by immunohistochemistry (IHC) in breast cancer tissues and the expression levels of ERα, PR and HerZ mRNA were detected by real-time qRT-PCR in breast cancer tissues and benign breast tumor tissues. The values of these two methods in diagnosis of breast cancer were evaluated. Results The expressions of ERα and HerZ mRNA were significantly higher in the breast cancer tissues than in the controls (P0. 05). Real-time qRT-PCR in detecting ERα, PR and Her2 mRNA expression had similar capability with IHC method in evaluating the sensitivity, specificity of endocrine thERαpy; moreover, the two methods also had a consistent pathological diagnosis rates (P>0. 05). Conclusion ERα, PR and Her2 genes are important predictive markers for endocrine thERαpy or targeted thERαpy of breast cancer. Real-time qRT-PCR method can be used for clinical detection and research of ERα, PR and Her2 mRNA.