Research on 5α, 8α-epidioxy sterols from the south china sea gorgonian menella kanisa / 第二军医大学学报

ABSTRACT

Objective To investigate the chemical components of gorgonian Menella kanisa collected from the South China Sea. Methods The Et2O extract of gorgonian Menella kanisa was purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The structures of the obtained compoundswere determined on the basis of spectroscopic analysis and compared with the reported data. Results and Conclusion Five 5α, 8α-epidioxy sterolswere isolated from gorgonian Menella kanisa collected from the South China Sea, and their structures were determined as 5α, 8α-epidioxy-cholest-6-en-3/β-ol (1), (22E)-5α, 8α-epidioxy-cholesta-6, 22-dien-3/β-ol (2), (22E, 2iR)-5α, 8α-epidioxy-24-methyl-cholesta-6, 22-dien-β-ol (3), 5α, 8α-epidioxy-24-methyl-cholesta-6, 24 (28)-dien-β-ol (4), (24E)-5α, 8α-epidioxy-24-ethyl-cholesta-6, 24(28)-dien-β-ol (5). All the five epidioxy sterols have been separated from the title Menella kanisa for the first time.