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Effect of adenovirus-mediated hepaCAM gene expression on cell cycle of bladder cancer cell lines / 第二军医大学学报
Academic Journal of Second Military Medical University ; (12): 1151-1154, 2013.
Artículo en Chino | WPRIM | ID: wpr-839492
ABSTRACT

Objective:

our study was to investigate the effect of hepaCAM gene on cell cycle of bladder cancer cell line T24 and BIU-87.

Method:

Adenovirus vector with hepaCAM was infected into T24 and BIU-87 cells. The expression of hepaCAM mRNA and protein were measured byRT-QPCR and Western-blot. Immunofluorescence detected the cellular localization of hepaCAM. The distribution of cell cycle was analyzed by FCM. The protein expression of cyclinD1 was measured by Westein-blot.

Result:

The expression of hepaCAM mRNA and protein was increased in two cell lines infected with pAdH5-hepaCAM. The G0/G1 cycle was increased in infected cells, and the protein expression of cyclinD1 was decreased in same cells. Immunofluorescence revealed that hepaCAM protein localized on cytoplasm in well-spread cells, otherwise it localized on the junction of each cell.

Conclusion:

T24 and BIU-87 cells that infected pAdH5- hepaCAM was blocked in G0/G1 cycle, and it can decreased the protein expression of cyclinD1.

Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Academic Journal of Second Military Medical University Año: 2013 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Idioma: Chino Revista: Academic Journal of Second Military Medical University Año: 2013 Tipo del documento: Artículo