Effect of serum of end stage renal disease patients undergoing different types of blood purification on ABCA1 expression in macrophages / 第二军医大学学报

ABSTRACT

Objective: To observe the effect of serum of end stage renal disease patients undergoing maintenance hemodialysis (MHD) on the expression of ATP-binding cassette transporter Al (ABCA1) in macrophages, and to study the interventional effects of different types of blood purification on the effect of serum. Methods: Totally 40 MHD patients were divided into hemodialysis (HD) group (n = 23) and hemodiafiltration (HDF) group (n=17), and 23 healthy people were taken as controls. THP-1 macrophages were incubated with sera of subjects from each group. The serum lipid profiles and hs-CRP were measured by automatic biochemical analyzer. The serum levels of TNF-α, monocyte chemotactic protein 1 (MCP-1) and IL-6 were measured by ELISA. The mRNA expression of ABCA1 in macrophage was examined by RT-PCR. Western blotting analysis was used to measure protein expression of ABCA1,STAT3 and p-STAT3 in THP-1 macrophages. Results: The levels of high-density lipoprotein cholesterol (HDL-c) and apolipoproteinA I (ApoA- I) in patients with end stage renal disease were significantly lower, and those of hs-CPR, TNF-α, MCP-1,and IL-6 were significantly higher than those in healthy group (P< 0. 05). The expressions of ABCA1, STAT3 and p-STAT3 in macrophages were not significantly different before treatment with HD and HDF, but the levels of ABCA1 in HD group and HDF group were significantly lower than those in healthy control group (P<0. 05). The levels of ABCA1, STAT3 and p-STAT3 were not significantly changed before and after treatment in HD group. In contrast, the levels of ABCA1, STAT3 and p-STAT3 were significantly increased after treatment in HDF group(P< 0. 05). Conclusion: HDF is more potent than HD in up-regulating ABCA1 in macrophages of MHD patients, enhancing its anti-inflammation and inducing cholesterol efflux, and the mechanism might be related to the activation of STAT3 signaling pathway.