Influence of edaravone on major organ functions and plasma inflammatory cytokine level following hemorrhagic shock and resuscitation in rats / 第二军医大学学报

ABSTRACT

Objective: To investigate the effects of edaravone on the functions of major organs and plasma inflammatory cytokine levels following hemorrhagic shock(HS) and resuscitation in rats. Methods: A total of 24 rats were randomly divided into 3 groups: the sham-operated control group (sham), the haemorrhage shock goup (I/R), and the haemorrhage shock plus edaravone injection (I/R-ED) group. Rats in the latter two groups were bled using a femoral artery catheter with MAP maintained within 35-45 mmHg(1 mmHg=0.133 kPa) for 60 min to induce shock, and then resuscitaton was induced by reinjecting 60% autologous blood and 2 times of shed blood Ringer's. At the beginning and ending of resuscitation, I/R group and I/R-ED group were given the same dose (2 ml/kg) of normal saline and edaravone, respectively. The plasma levels of TNF-α, IL-6, ALT, AST, LDH, CK, BUN and Cr were measured at 2 and 24 h after resuscitation. Results: (1)The plasma levels of TNF-α and IL-6 in I/R group were significantly higher than those of the sham group 2 h after hemorrhagic shock and resuscitation (P0.05). The plasma levels of TNF-α and IL-6 in I/R group were also significantly higher than those in the sham group after 24 h(P<0.05), and the levels in I/R-ED group was significantly lower than those in the I/R group(P<0.05). (2) The plasma levels of ALT and AST in I/R group were significantly higher than those in the sham group at 24 h (P<0.05), and the levels in I/R-ED group was significantly lower than that in the I/R group (P<0.05). The plasma levels of BUN and Cr in I/R group were significantly higher than those in the sham group after 2 h(P<0.05), and the levels in I/R-ED group was significantly lower than those in the I/R group(P<0.05). The plasma levels of BUN and Cr in I/R group were also significantly higher than those in the sham group (P<0.05); Cr level in ED group was significantly lower than that in the I/R group(P<0.05); and the BUN level in I/R-ED group was similar to that in I/R group. (3) Great pathological changes were found in the liver, kidney, and lung of rats in I/R Group, and the pathological changes were slight in I/ R-ED Group. Conclusion: Our study suggests that edaravone can decrease the release of inflammatory factors (TNF-α and IL-6) after induction of HS in rats, which can alleviate the pathological damage to major organs after HS.