Improvement in specimen preparation of ultra-thin section staining for transmission electron microscopy / 第二军医大学学报

ABSTRACT

Objective: To improve the staining efficiency and quality of biological specimens ultra-thin section for transmission electron microscopy. Methods: The "plug-in staining method" and continuous staining were developed by modifying the traditional staining method. Results: Using our method, we completed the electron staining of a large number of ultra-thin sections of biological specimens of within a short period of time. Conclusion: Compared with traditional method, the new method can save time and drug, and reduce the possibility of staining contamination. The ultrastructure observed by electron microscope is more clear and distinct.