Effect of aerosolized pulmonary surfactant on blood oxygenation in rats with acute lung injury / 第二军医大学学报

ABSTRACT

Objective: To investigate the effect of aerosolized pulmonary surfactant (PS) on the blood oxygenation in rats with acute lung injury(ALI). Methods: Forty-three SD rats were randomly divided into 4 groups sham group (operated only, n = 10), model group(n=10), air-NS group (inhaling aerosolized normal saline, n=10), and air-PS group (inhaling aerosolized PS[160 mg], n=13). ALI rats were induced by oleic acid(0.2 ml/kg) through jugular vein. The arterial blood gas and breathing rates were determined during the experiment in all groups. The survival rates of all groups were determined after experiments. Results: PS aerosolization led to a gradual increase of PaO2 and SaO2. Four hours after inhalation of aerosolized PS, the PaO 2 and SaO2 increased to 99.4 mmHg(1 mmHg=0.133 kPa) and 98.2%, respectively, which were significantly higher than those in the air-NS group (P< 0.05). The breathing rate began to decease 0.5 h after inhalation of aerosolized PS and decreased to 100 breaths/min 4 h later, which was significantly lower than that in the air-NS group at the same time point (P<0.05). The survival rate of animals in the air-PS group was 100%, also significantly higher than that in the air-NS group (60% , P<0.05). Conclusion: Aerosolized PS through spontaneous breathing can improve the blood oxygenation, pulmonary function and survival rates of ALI rats.