Role of JNK phosphorylation in intestinal ischemia/reperfusion injury in mice / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12): 140-143, 2010.
Artículo
en Chino
| WPRIM
| ID: wpr-840370
ABSTRACT
Objective:
To explore the role of c-Jun NH2 terminal kinase (JNK) activation and JNK-mediated apoptotic signal pathway in intestinal ischemia/reperfusion (II/R) in mice.Methods:
C57BL/6 mice were randomly divided into sham-operated group (n = 6) and II/R groups (n = 36); the latter was further divided according to time after perfusion (0,0. 5,1,4,6 and 12 h). Animal II/R model was established by clamping the superior mesenteric artery (SMA) for 40 min followed by reperfusion. Animals in the sham-operated group received no clamping. Animals in the two groups were sacrificed at defined time points, and the expression of JNK, phosphorylation (phospho-) JNK, cleaved caspase-3,Bcl-2 and Bax protein in the intestinal tissue was examined by Western blotting analysis, and the pathological changes of ileum tissue were observed under optical microscope.Results:
Most severe intestinal injury was found at the early stage of reperfusion, and the intestinal tissues almost recovered 12 h later. The phospho-JNK in the intestine was significantly elevated within 1 h after II/R compared with sham group (P<0. 01). Cleaved caspase-3 was significantly increased in II/R group at 0. 5 h, 1 h after reperfusion compared to sham group (P<0. 01); the expression of Bcl-2 protein in II/R group was significantly decreased compared with the sham-operated group (P<0. 01), and there was no significant difference in Bax expression between different groups.Conclusion:
JNK phosphorylation plays an essential role in the intestinal damages induced by II/R,possibly through down-regulating Bcl-2 protein expression and caspase-3 dependent apoptosis pathway.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Academic Journal of Second Military Medical University
Año:
2010
Tipo del documento:
Artículo
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