Adoptive immunotherapy with CTL induced by PSA-pulsed dendritic cell vaccine for prostate cancer in mice / 第二军医大学学报

ABSTRACT

To observe the anti-tumor effect of adoptive immunotherapy with CTL induced by PSA-pulsed dendritic cell vaccine against prostate cancer in mice. Methods: Nude mice were s. c. inoculated with LNCaP tumor cells to establish prostate cancer model. CTL induced in vitro by different peptide-pulsed dendritic cells (Non-DC,Ova-DC,Lys-DC and PSA-DC) were used for adoptive immunotherapy of experiment nude model through vena caudalis on d 15 and d 22 after inoculation. The survival of nude mice and the sizes of tumors were observed with d 50 after the initial treatment as the endpoint. Results: The tumor sizes in Non-DC, Ova-DC, Lys-DC and PSA-DC groups on d 30 and d 50 after adoptive immunotherapy were significantly larger than that at d 15 after inoculation (P<0. 01). The tumors sizes in Lys-DC and PSA-DC groups were significantly smaller than those in the Non-DC and Ova-DC groups(P<0. 01). The survival periods of animals in Lys-DC and PSA-DC groups were significantly longer than those in the Non-DC and Ova-DC groups (P<0. 01). Conclusion: Adoptive immunotherapy with CTL induced by DC-based PSA vaccine can inhibit the growth of prostate cancer LNCaP cells in nude mice and increase the survival of the animals.