Stability of rh-endostatin and fabrication/release test of rh-endostatin loaded PLGA microspheres / 第二军医大学学报

ABSTRACT

Objective: To prepare rh-endostatin loaded poly (lactic-co-gtycolic acid) (PLGA) microspheres and to evaluate their release behavior in vitro. Methods: Rh-endostatin PLGA microspheres were prepared by W/O/W process. The content and in vitro cumulative release was determined by a HPLC method. Results: The prepared microspheres were well-shaped, with a mean diameter of 122.7 μm. The drug loading and encapsulation efficiency were 1.28% and 38.65%, respectively. The rh-Endo solution (250 μg/ml) showed good stability after placed at 4°C and 25°C for 108 h. The cumulative in vitro release was up to 67.37% in 28 days. Conclusion: The rh-Endo can be encapsulated in microspheres to yield sustained release using biodegradable polymers PLGA as the carrier material.