Cytoplasmic domain of leukemia inhibitory factor receptor regulates proliferation and differentiation of leukemia cells / 第二军医大学学报

ABSTRACT

Objective: To investigate the effect of the cytoplasmic domain of leukemia inhibitory factor (LIF) receptor α submit, gp190CT3, on activation of the JAK/STAT3 signal transduction pathway and on promotion of leukemia cell HL-60 differentiation into granulocytes. Methods: pcDNA3, 0-gp190CT3 was used to transfect CHO cells. Using immunofluorescent cytochemistry, RT-PCR and flow cytometry techniques, we detected the expression of gp190CT3 gene and protein in the stably-transfected CHO cells. Then the stably-trasfected CHO cells were co-cultured with HL-60 cells and the morphological changes of the HL-60 cells were observed, the levels of STAT3 phosphorylation and the expression of the cell surface antigen CD15 were also determined. Results: Expression of gp190CT3 gene was found in pcDNA3. 0-gp190CT3 transfected CHO cells and gp190CT3-stably-transfect CHO cells were obtained. Compared with the untreated HL-60 cells, the size of the co-cultured HL-60 cells was increased, the morphology was irregular and the level STAT3 phosphorylation and the expression of CD15 were increased. Conclusion: The LIF receptor α submit gp190CT3 participates in the activation of JAK/STAT3 signal transduction pathway and regulates HL-60 cell differentiation and proliferation.