Physicochemical property and in vitro antitumor activity of arsenic trioxide albumin microspheres / 第二军医大学学报

ABSTRACT

Objective: To prepare arsenic trioxide (As2 O3) albumin microspheres and to analyze their physicochemical properties and in vitro antitumor activities. Methods: As2 O3 albumin microspheres were prepared by emulsification-heat solidification. Physicochemical and the slow-release properties of the microspheres were identified by the electron microscope, thermal stabilization analysis and dynamic dialysis system in vitro. MTT method was used to evaluate the inhibitory effect of the released fluid on human osteosarcoma cell line U-2 OS cells and human lung adenocarcinoma cell line SPC-A-1 cells. Results: The mean diameter of the microspheres was (156 ± 3.56) μm (ranging 56. 4-256. 7 μm), with the AS2 O3 content being (55.22±11.19)% and t1/2 of release time being 23.34 h. The slowly released fluid showed a significant inhibitory effect on U-2 OS and SPC-A-1 cells. Conclusion: As2 O3 albumin microspheres prepared in the present study have satisfactory physicochemical, slow-releasing, and tumor-inhibition properties.