Auto-bone marrow mononuclear cells transplantation accelerates reendothelialization and inhibits neointima formation in vein grafts / 第二军医大学学报

ABSTRACT

Objective: To assess the effect of auto-bone marrow mononuclear cells (BMMNCs) transplantation on reendothelialization and neointima formation in vein grafts. Methods: BMMNCs were extracted from the bone marrows of adult rabbit under sterile environment and were labelled with DAPI before transplanted into vein grafts. Twenty adult rabbits were randomly divided into 2 groups BMMNCs transplantation group(group I, n 10) and PBS transplantation group (group II, n=10). The left external jugular vein of 20 rabbits were harvested and transplanted between ipsolateral common carotid artery (AVG). Three days later, animals in group I were transplanted with BMMNCs(6x108 cells)/100 μl via periotic veins and those in group II were injected with 100 μl PBS. Animals were killed 4 weeks later and graft veins were harvested to observe the reendothelialization and the thickness of vein grafts. Results: We found that the transplanted cells survived and were incorporated into the endothelium of vein grafts in group I. The endothelium integrity of the vein grafts in group I was significantly better than that of group II. The intima thickness of vein grafts in group I was significantly thicker than that of group II. Conclusion: BMMCs transplantation therapy may improve re-endothelialization of the vein graft and inhibits intimal hyperplasi the vein graft.