Triplex forming oligonucleotide of PDGF-B chain combined with antisense oligonucleotide of VEGF inhibits glioma growth in rats / 第二军医大学学报

ABSTRACT

Objective: To observe the inhibitory effect of triplex forming oligonucleotede (TFO) of PDGF-B chain combined with antisense oligonucleotide (AON) of VEGF on glioma growth in rats. Methods: Thirty -six male SD rats received 1×106 C6 glioma cells/20 μl normal saline into the right caudate putamen by stereotactic technique. Group I (n=6) was treated in situ with 1.5 mg/20 μl TFO for 3 times on 8,11 and 14 d after glioma cell inoculation. Group II (n=12) and III (n=12) were respectively treated with TFO 1.5 mg+AON 0.125 mg/20 μl and TFO 1.5 mg+AON 0.250 mg/20 μl for 3 times on 8,11 and 14 d after glioma cell inoculation. Control group (n=6) was treated with 20 μl saline for 3 times at the same time pionts. Three weeks after cell inoculation, all rats were sacrificed to observe tumor growth and to determine the expression of PDGF-B,VEGF and PCNA. Results: The inhibiti on rate of tumor growth was 53.1% in group I, 81.4% in group II and 93.1% in group III, with significant difference found between the 3 groups (P<0.01). PDGF-TFO had obvious inhibitory effect on the expression of PDGF-B, VEGF and PCNA in C6 glioma cells;and PDGF-TFO combined with VEGF-AON had more potent inhibitory effect on the expression of PDGF-B, VEGF and PCNA in C6 glioma cells. Conclusion: PD GF-TFO combined with VEGF-AON has more potent inhibitory effect on tumor growth than PDGF-TFO alone.