Effects of oxaliplalin combined with capecitabine on expressions of tumor-related factors in gastric cancer tissue of rats with experimental gastric cancer and their synergistic anti-tumor effects / 吉林大学学报(医学版)

ABSTRACT

Objective: To investigate the effects of oxaliplatin combined with capecitabine on the expressions of tumor-related factors in the gastric cancer tissue and the levels of serum inflammatory factors in the rats with experimental gastric cancer, and to elucidate the possible mechanism of their anti-tumor effects. Methods: Sixty Wistar rats were randomly divided into control group∗ model group∗ oxaliplatin group∗ capecitabine group and oxaliplatin combined with capecitabine group (combination group); there were 12 rats in each group. The BGC823 cells were used to establish the experimental gastric cancer models. The experimental rats were given capecitabine (400 mg • kg ) and (or) oxaliplatin (20 mg • kg ) at the same time; the rats in control group and model group were given the normal saline at the same volume for 8 weeks. The average tumor weight was measured after administration. The apoptosis of gastric cancer cells was measured by TUNEL staining. The protein expression levels of P53. signal transduction and activation factor 3 (STAT3) and vascular endothelial growth factor (VEGF) in the gastric cancer tissue of the rats in various groups were detected by Western blotting method. The levels of serum interleukin-6 (IL-6)» interleukin-10 (IL-10) and tumor necrosis factor-a (TNF-a) of the rats in various groups were detected by ELISA. Results: Compared with control group, the protein expression levels of P53» STAT3. and VEGF in the gastric cancer tissue and the levels of serum IL-6. 11,-10. and TNF-a of the rats in model group were significantly increased (P<0. 05). Compared with model group, the average tumor weights and the protein expression levels of P53» STAT3. and VEGF in the gastric cancer tissue and the levels of serum IL-6. IL-10. and TNF-a of the rats in oxaliplatin group, capecitabine group and combination group were significantly decreased (P∗C0. 05); the apoptosis indexes (AI) of gastric cancer cells were significantly increased (P<0. 05). The average tumor weight, the protein expression levels of P53∗ STAT3∗ and VEGF in gastric cancer tissue and the levels of serum IL-6. IL-10. and TNF-a of the rats in combination group were significantly lower than those in capecitabine group and oxaliplatin group ( P-C0. 05). Conclusion: Both oxaliplatin and capecitabine can paly the antitumor effects by reducing the protein expression levels of the tumor-related factors and the serum IL-6. IL-10. and TNF-a levels of the gastric cancer rats, and the anti-tumor effect of combined utilization is better than that of capecitabine or oxaliplatin used alone.