Prostagladin E Receptor II and IV Increase the Expression of Martrix Metalloproteinase-7 in PC (Prostate Cancer)-3 Cells / 대한비뇨기과학회지
Korean Journal of Urology
;
: 478-484, 2004.
Artículo
en Coreano
| WPRIM
| ID: wpr-84248
ABSTRACT
PURPOSE:
The effects of PGE2 receptors (EP1, 2, 3, 4) on the proliferation of prostate cancer cells are still unclear. The degradation of the basement membrane by MMP-2, 7, 9 and TIMP-1, 2 is a critical point in tumor invasion and metastasis. We investigated the effects of PGE2 receptors concerning MMP and TIMP after the treatment of COX-2 inhibitors on prostate cancer cell-lines. MATERIALS ANDMETHODS:
Two prostate cancer cell-lines, PC-3 and DU-145 cells were used in this study. RT-PCR were performed to detect the mRNA expression of EP1, 2, 3, 4, MMP-2, 7, 9 and TIMP-1, 2, MMP-7 was measured by ELISA after being treated with the selective EP2 agonist and EP4 agonist 10(-10), 10(-8), 10(-6) microM respectively.RESULTS:
EP2, 3 and 4 mRNA were expressed in both cell-lines. After the NS-398 treatment, EP2 and EP4 mRNA expressions decreased in PC-3 cells. While only the MMP-7 mRNA expression decreased in PC-3 cells after NS-398 treatment, after NS-398 with selective EP2 agonist and EP4 agonist, MMP-7 mRNA expression increased. In PC-3 cells, selective EP2 agonist and EP4 agonist induced a significant dose-dependent increase in MMP-7 production in comparison to the NS-398 treatment group (control) in the conditioned ELISA medium.CONCLUSIONS:
These results strongly suggest that COX-2, to some extent, contribute to prostate carcinogenesis at the EP2 and EP4 receptor, which could also be explained by increments of MMP-7 in PC-3 cells. Therefore, these findings show that selective EP inhibitor is useful in preventing specific disease progression in prostate cancer.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Asunto principal:
Próstata
/
Neoplasias de la Próstata
/
Membrana Basal
/
ARN Mensajero
/
Ensayo de Inmunoadsorción Enzimática
/
Prostaglandina-Endoperóxido Sintasas
/
Receptores de Prostaglandina E
/
Progresión de la Enfermedad
/
Inhibidor Tisular de Metaloproteinasa-1
/
Metaloproteinasa 7 de la Matriz
Idioma:
Coreano
Revista:
Korean Journal of Urology
Año:
2004
Tipo del documento:
Artículo
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