Fabrication of an antibacterial hydrogel laden with adhesive liposomes for bone repairment / 上海交通大学学报（医学版）

ABSTRACT

Objective: To construct an antibacterial hydrogel laden with adhesive liposomes (A-LIP-PEG) for bone repair. Methods: Adhesive liposomes (A-LIP) were fabricated based on the Schiff-based reaction between dopamine and the liposome. To prepare the liposome composite hydrogel, the A-LIPs were mixed with thiolated polyethylene glycol (4SH-PEG) which was then crosslinked with Ag+. Bone morphogenetic protein 2 (BMP-2) was loaded in the A-LIPs for its uniform dispersion and sustained release in the hydrogel network. The size distribution and zeta-potential of the A-LIPs were characterized with a particle size analyzer. The morphology of the A-LIPs was observed under transmission electron microscope. Flushing test was employed to examine the viscidity of the A-LIPs, and CCK-8 assay was conducted to demonstrate the biocompatibility of the A-LIP-PEG. Osteogenic activity of the A-LIP-PEG was evaluated by alkaline phosphatase assay and alizarin red S staining in bone marrow mesenchymal stem cells (BMSCs). Results: Strong adhesion toward soft tissue of the A-LIPs was indicated by in vitro study. A-LIP-PEG showed significant inhibition on Staphylococcus aureus. A-LIP-PEG showed superior promotion of osteogenic differentiation to hydrogel laden with liposomes (LIP-PEG), without disrupting cell proliferation. Conclusion: The A-LIP-PEG developed in this study shows notable potential to expand the application of hydrogels to drug delivery, antibacteria and bone repair.