Promotion of 640 nm red light on keratinocyte migration via CD26 / 上海交通大学学报(医学版)
Journal of Shanghai Jiaotong University(Medical Science)
;
(12): 843-847, 2019.
Artículo
en Chino
| WPRIM
| ID: wpr-843374
ABSTRACT
Objective:
To investigate the mechanism of 640 nm red light promoting keratinocyte migration preliminarily.Methods:
Human keratinocytes were cultured in vitro and assigned to 4 groups according to different interventions, i.e. control group, sitagliptin group (CD26 expression inhibited), 640 nm red light group (8 J/cm2) and 640 nm red light+sitagliptin group. The levels of CD26 mRNA and protein in keratinocytes were measured with realtime-PCR and Western blotting, respectively. Cell migration was observed with Transwell assay and scratch assay.Results:
The level of CD26 mRNA and the expression of CD26 protein in 640 nm red light group obviously increased compared with control group, while those of sitagliptin group significantly decreased. Migration ability of keratinocytes increased in 640 nm red light group and decreased in sitagliptin group observed by Transwell assay and scratch assay.Conclusion:
The expression of CD26 in keratinocytes is promoted by the 640 nm red light, which can accelerate keratinocyte migration to facilitate re-epithelialization.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Shanghai Jiaotong University(Medical Science)
Año:
2019
Tipo del documento:
Artículo
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