Function of sahH gene in exopolysaccharides synthesis of Streptococcus mutans LuxS null strain / 上海交通大学学报(医学版)
Journal of Shanghai Jiaotong University(Medical Science)
;
(12): 254-258, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-843746
ABSTRACT
Objective:
To investigate the function of sahH gene in exopolysaccharides synthesis of Streptococcus mutans LuxS null strain.Methods:
sahH+luxS-SmUA159 was constructed by introducing sahH gene into Streptococcus mutans LuxS null strain, while PIB169+luxS-SmUA159 and its wild strain SmUA159 were used as vector control and blank control. Real-time qPCR and anthrone test were performed to investigate the differences in exopolysaccharides synthesis among sahH+luxS-SmUA159, PIB169+luxS-SmUA159 and the wide-type strain SmUA159.Results:
The transcriptional levels of gtfA and gtfD were upregulated in the sahH+luxS-SmUA159, compared to SmUA159 and PIB169+luxS-SmUA159 (both P<0.05), but there was no statistical difference in the transcriptional levels of gtfB and gtfC. The results of the anthrone test showed exopolysaccharides synthesis of the three groups was also similar.Conclusion:
sahH gene affects the expression of some genes related to exopolysaccharides synthesis, but it has little effect on the whole content of bacterial exopolysaccharides.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of Shanghai Jiaotong University(Medical Science)
Año:
2018
Tipo del documento:
Artículo
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