The expression and biological function of SOX12 in oralsquamous cell carcinoma by bioinformatics analysis / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the expression of transcription factor SOX12 in oral squamous cell carcinoma (OSCC) by bioinformatics analysis and to clarify the connection between expression of SOX12 and clinicopathological features of OSCC, so as to elucidate the early diagnosis and prognostic significance of OSCC by SOX12. Methods:This study used the GEPIA database in conjunction with the TCGA database to analyze the expression level of SOX12 in OSCC tissues and then verify it by qRT-PCR in vitro. The LinkedOmics database was used to describe the correlation between SOX12 expression and clinical pathological parameters of OSCC and its impact on prognosis. The location of SOX12 in signal transduction and its upstream and downstream genes related to it were analyzed by the String-DB database. Results: SOX12 was highly expressed in OSCC and positively correlated with OSCC pathological grade, T stage, and N stage. The results of TCGA showed that high SOX12 mRNA expression was associated with overall survival in OSCC. The proteins interacting with SOX12 included POU3F1, POU3F2, POU3F3, MYT1L, and NRSN2, which were mainly involved in the differentiation and development of neurons. Conclusion: The TCGA database analysis found that SOX12 is highly expressed in OSCC and is associated with multiple pathological indicators of OSCC.