Relationship of clustered regularly interspaced short palindromic repeats with virulence genes and resistance genes in Shigella / 西安交通大学学报(医学版)

ABSTRACT

Objective: To investigate the relationship of the clustered regularly interspaced short palindromic repeats (CRISPR) with resistance genes and virulence genes. Methods: The Shigella genome sequences, resistance genes and virulence genes sequences were obtained from GenBank Database. Then we obtained the distribution of CRISPR1, CRISPR2 and CRISPR-Q1 and the information of repeat and spacers in Shigella by multiple sequences alignment. Meanwhile, the distribution of resistance genes and virulence genes in Shigella were obtained by BLAST and the relationship of the CRISPR with resistance genes and virulence genes was computed by SPSS 17.0. Results: The spacers of CRISPR1 and CRISPR2 showed obvious differences in different Shigella strains, and the CRISPR-Q1 was more widely distributed (over 99%). The virulence genes were widely distributed in Shigella (more than 56%); the distribution of resistance genes (blaOXA-1, camr, Sul2, and tetB) was also wide (over 50%). There were significant differences between the CRISPR1/CRISPR2 and 9 kinds of virulence genes and 4 kinds of resistance genes (P<0.05). There were significant differences between the CRISPR-Q1 and 3 kinds of virulence genes and 4 kinds of resistance genes (P<0.05). There were significant differences between the CRISPR1/CRISPR2, CRISPR-Q1 and the number of virulence genes and resistance genes (P<0.05). There were significant differences between the CRISPR1/CRISPR2 and the number of spacers of CRISPR-Q1 (χ2=61.6, P<0.001). Conclusion: The distribution of CRISPR was quite different in Shigella, and the number of spacers was also different. There were negative correlation of CRISPR of Shigella with some resistance genes and virulence genes. The CRISPR1/CRISPR2 had a positive relationship with the number of the spacers.