Preparation and identification of thyroid gland decellularized biologic scaffold / 解剖学报

ABSTRACT

Objective: To prepare thyroid gland decullularized biologic scaffolds by soaking with rotating and to assess the decullularized scaffolds. Methods: According to random number table method, twenty New Zealand white rabbits were divided into control group and decullularized group, 10 cases in each group. The thyroid glands in decullularized group were immersed in 1% SDS and rotated at 37°C for 24 hours. All thyroid gland decullularized scaffolds were rotated in deionized water to wipe off the decullularized reagent and residual DNA. HE staining, DNA concentration examination, electron microscope analysis and immunofluorescence technique were performed to indentify the characteristics of decullularized scaffolds. Results: Compared to control group, the DNA concentration in the decellularized scaffold decrease significantly (P<0.01), the removal rate of DNA exceeded 90%; there were no visible cell nuclei remained, the 3D space structure and the protein components preserved well. Conclusion: Soaking and rotating method can effectively wipe off the cellular components from the thyroid gland tissue, and preserve the extracellular matrix and three-dimensional structure.