Inhibition effect of sorafenib on adjuvant arthritis in rats / 解剖学报

ABSTRACT

Objective: To observe the inhibition effect of sorafenib on adjuvant arthritis in rats. Methods: A total of 36 male SPF SD rats were divided into 6 groups and 0. 1 ml of the complete Freund' s adjuvant was subcutaneously injected into the left hind paw except the normal group. The volume of rat hind paw was measured. The CD4+ and CD8+ T cell subsets of the peripheral blood were analyzed by flow cytometry. The microvessel density of synovial tissues was determined by immunohistochemical chain mildew avidin peroxidase enzymatic (SP) method. Results: Compared with model group, the rats of sorafenib groups alleviated the volume of rat hind paw and reduced microvessel density. Sorafenib (20, 40mg/kg) groups decreased the percentage of CD4+ T cell and increased the percentage of CD8+ T cell at the same time. All the values were statistically significant (P < 0. 05). Conclusion: Sorafenib can ameliorate adjuvant arthritis in rats, which effect may be related to sorafenib causing CD4+ and CD8+ T cell subset of peripheral blood deviation and reducing microvessel density of synovial tissues.