Determination of the release of TH-302 hepatic artery embolic microspheres in vitro by HPLC / 国际药学研究杂志
Journal of International Pharmaceutical Research
;
(6): 320-324, 2018.
Artículo
en Chino
| WPRIM
| ID: wpr-845355
ABSTRACT
Objective:
To establish a high performance liquid chromatography(HPLC)method for the determination of the release of TH-302 hepatic artery embolization microspheres in vitro.Methods:
The release of TH-302 hepatic artery embolization mi- crospheres in vitro was determined by HPLC. The Venusil ASB C 18 column(4.6 mm×150 mm,5 μm)was used. The mobile phase con- sisted of acetonitrile-water(7030,V/V)with a flow rate of 1 ml/min,and the detection wavelength was 316 nm. The column tempera- ture was 30℃ and the injection volume was 10 μl.Results:
The quantification limit and detection limit were 1.4 and 0.4 μg/ml,re- spectively. The calibration curves were linear within the range of 0.1-10 μg/ml,and the linear equation was Y=10.867X+0.3034(R 2 = 1.0000,n=8). For concentration,the average recovery was within the range of 98.99%-101.77%(n=9)(RSD%=0.07%). The stability results showed that TH-302 was unstable under long-term release conditions,therefore,a linear relationship between the actual degra- dation peak area and the theoretical degradation content of TH-302 was established to calculate the microsphere release accurately. The linear equation was A actual = 0.0523M degredation - 1.4166(R 2 =0.9919).Conclusion:
The method could be used for the determination of the release of TH-302 hepatic artery embolization microspheres,which is convenient,fast,sensitive and reproducible,with good pre- cision,specificity and accuracy.
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Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Journal of International Pharmaceutical Research
Año:
2018
Tipo del documento:
Artículo
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