Effects of ento-i plastic on anti-anoxia and myocardial ischemia in mice: A preliminary study / 国际药学研究杂志

ABSTRACT

Objective To investigate the effects of Ento-I plastic on anoxia and myocardial ischemia. Methods The effect of Ento-I plastic on anoxia in mice was evaluated by testing mice’s anti-anoxia time under ordinary pressure, mice’s gasp time and the number of mouth breathing under ischemia-anoxia, and mice’s survival time after sodium nitrite poisoning. Animals were divided into normal saline(NS)group, matrix group, aspirin(ASP)group and Ento-I plastic(9.125, 18.25 and 37.5 mg/kg)groups. The effect of Ento-oon myocardial ischemia in mice was preliminarily evaluated using the animal model of acute myocardial ischemia induced by the subcutaneous injection of isoprenaline(the animals were divided into NS group, matrix group, compound danshen dropping pill(DSP)group and Ento-I plastic(5, 10 and 20 mg/kg)groups. In these experiments, NS group, ASP group and DSP group were administered intragastrically, while the matrix group and Ento-I plastic groups were administered by daubing on both sides of the temple. Results There was no significant difference in all indexes between the NS group and the matrix group in all four experiments. Compared with NS, the middle dosage of Ento-I plastic(18.25 mg/kg)could obviously prolong the anoxia tolerance time to (50.87±11.90)min under ordinary pressure, survival time to(14.15±4.61)min after sodium nitrite poisoning and the gasp time to (27.90±4.79)s after decapitation, with the statistically significant difference(P<0.01). In anti-myocardial ischemia experiment, both the myocardial superoxide dismutase(SOD)activity and malondialdehyde(MDA)content were significantly decreased in the high dosage Ento-I(20 mg/kg)and DSP groups than in the NS group(P<0.01). The pathological examination of the ischemic of myocardium itself showed that Ento-I plastic could significantly reduce ischemic myocardial injury and enhance the anti-ischemic effect of myocardium itself. Conclusion The Ento-I plastic could obviously strengthen the anti-anoxic and anti-ischemic ability of mice.