Optimization of the extraction process of oleanolic acid in Ligustrum lucidum fruits by macroporous resin purification / 国际药学研究杂志

ABSTRACT

Objective To optimize the extraction and purification process of oleanolic acid in Ligustrum lucidum fruits with macroporous resin. Methods Grinding degree, temperature, liquid ratio, extraction time, extraction times, ethanol volume fraction and pH were selected as factors, which may influence the extraction of oleanolic acid from L. lucidum. Single factor experiments were carried out on these factors. The optimal extraction conditions were determined, and the best type of resin was chosen for separation and purification of oleanolic acid in the optimal conditions. Results The optimal technological conditions were as follows the temperature was controlled at 80°C, ethanol solution was 60%, liquid material ratio was 111, extraction time was 120 min, and extraction times were twice. The crude extraction was processed at pH 12, and then the pH was adjusted to 2 to further purify the processed crude extraction. The content of oleanolic acid in the extract was 9.5% by UV visible spectrophotometric method. This extraction was further separated and purified by AB- 8 resin, and the content of oleanolic acid detected by ultraviolet visible spectrophotometr was 15.2%o Conclusion The optimal process of the oleanolic acid extraction is reproducible, simple and feasible. It may provide reference for using macroporous resin to extract oleanolic acid from L. lucidum in the industry production.