Identification and quantification of γ-aminobutyric acid in Ramulus Mori by TLC and HPLC / 国际药学研究杂志

ABSTRACT

Objective To establish the methods for identification and quantification of γ-aminobutyric acid (GABA) in Ramulus Mori by TLC and HPLC. Methods GABA was extracted by ultrasonic method with ethanol as solvent. The sample was applied on an efficient silica gel G plate with n-butanol-acetic acid-water (4∶2. 2∶1) as the developing system, and ninhydrin was used as chromogenie reagent. The content of γ-GABA was determined by HPLC. The separation was carried out on a ODS (4. 6 mm×250 mm, 5 μm) column with phosphate buffered solution (pH=6. 8)-methanol as a mobile phase with gradient elution, flow rate was 1. 0 ml/min, the detection wavelength was 335 nm. Results The TLC spots of GABA in Ramulus Mori were clear with good separation. The assay of GABA was linear in the range of 1. 006-84. 504 μg/ml and correlation coefficient was 0. 9994. Conclusion The methods are simple, accurate and reproducible which are valuable to identify Ramulus Mori.