Anti-tumor activity of long-circulation thermosensitive liposome-loaded vincristine / 国际药学研究杂志

ABSTRACT

Objective To investigate the anti- tumor activity of long- circulation thermosensitive liposom- loaded vincristine (VTSL) in vivo and in vitro. Methods The inhibitory effects of VTSL and vincristine injection (VCR) on sw620 cell and PANC cell were detected by MTT assay. The uptake capacity of HT-1080 was studied by using Cou-6-loaded liposome. Different xenograft nude mice models of HepG-2 and MCF-7 were established. To study anti-tumor effect of VTSL, drugs were given via tail and heat therapeutic area for 30 minutes, mice weight and tumor weight were recorded. To study anti-tumor effect of VTSL, drugs were given via tail vein and heat therapeutic areas for 30 min, mice body mass and tumor mass were recorded. Results After VTSL was given 72 h, the activity of sw620 and PANC was less than 5% and 20%, respectively. VTSL showed stronger cytotoxic effect than vincristine. At the same dose, tumor inhibitory rate of VCR and VTSL on HepG-2 and MCF-7 bearing nude mice was 50%, 69.7%, 47.8% and 76.1%, respectively. There were significant differences in tumor weight after treatment. Conclusion VTSL enhances the cytotoxicity by heating. Loading vincristine into TSL increased cytotoxicity, and heating could promote the fusion of liposomes and cell membrane. Under the same dosage, VTSL showed much higher tumor inhibition rate than VCR, and there was a certain dose dependence. The results show that VTSL can be used in treatment of solid tumor and has the potential to expand vincristine clinical application.