A comparative study on antidiarrheal effects of Mongolian medicines Holarrhena antidysenterica, Forsythia suspensa and Cynanchum thesioides / 中草药

ABSTRACT

Objective: To compare the antidiarrheal effects of Mongolian medicine, Holarrhena antidysenterica, Forsythia suspensa and Cynanchum thesioides on diarrhea model rats and investigate its effects on serum DAO (diamine oxidase), cAMP (cyclic adenosine phosphate), TNF-α (tumor necrosis factor-α), ATPase and calcium ions. Methods: The normal control group, model group, H. antidysenterica low-dose and high-dose groups, F. suspensa low dose and high-dose groups, C. thesioides low dose and high dose groups were set. Except the normal control group, the other groups were ig administrated water decoction of Cassia angustifolia to establish diarrhea model; After the success of the model, the rats in treatment groups were administrated by gastric drug for 7 d, the type mental state, diarrhea and body weight changes were observed. the abdominal aortic blood was obtained at the last day of fasting 12 h after the administration. DAO, cAMP, TNF-α, ATP enzyme, OD value of the calcium ions in serum were determined by using ELISA (enzyme-linked immune detection reagent) kits. Results: General status except for the normal group, the mental state of the rats in the other groups was depressed after modeling, the fur color of them was significantly decreased and the body weight was decreased. The diarrhea rate was 100% on the 4th day after modeling. Compared with the model group, there were significant differences in the number of loose stools, grade of loose stools and diarrhea index (P < 0.05, 0.01) in each administration group. The serum DAO, TNF-α, ATPase, cAMP and calcium ion OD values were compared the serum concentrations of DAO in H. antidysenterica low dose group, C. thesioides low dose group and F. suspensa high and low dose groups of rats were significantly lower than model group with significant differences (P < 0.05), and were significantly higher than normal group. The serum concentration of TNF-α in C. thesioides high and low dose groups were lower compared with model group (P < 0.05). The serum ATPase in C. thesioides high and low dose groups had significant difference (P < 0.05) compared with model group. The serum concentration of cAMP in H. antidysenterica high-dose group and F. suspensa low-dose group was significantly lower compared with model group with significant differences (P < 0.05). Serum Ca2+ concentration in the drug administration groups was significantly different from that in the model group (P < 0.05). Conclusion: The antidiarrheal effect of C. thesioides is better than that of H. antidysenterica and F. suspensa.