Regulatory effects of Cannabis Fructus oil on gut microecology in D-galactose- induced aging mice / 中草药

ABSTRACT

Objective: To investigate the effects of Cannabis Fructus oil on the regulation of gut microecology in D-galactose-induced aging mice. Methods: The Kunming mice were divided into control group, model group, positive control group (Jin Shuangqi group), Cannabis Fructus oil (12.0, 6.0, 3.0 mL/kg) group with six males and six females in each group. In the control group, normal saline was injected subcutaneously into the back of the neck every day. The other five groups were injected subcutaneously with 1% D-galactose aqueous solution, and the volume was 10 mL/kg. After 1 h, the control group was given normal saline by intragastric administration. The other groups were intragastrically administered with different doses of Cannabis Fructus oil for 42 d, and the dosage volume was 20 mL/kg. After the end of the administration, the change in body weight was analyzed; The proximal intestinal tissue of the ileocecal area and the feces in the cecum and colon were retained. Gram staining was used for the detection of Gram-positive bacilli (G+b), Gram-negative bacilli (G-b), Gram-positive cocci (G+c) and Gram-negative cocci (G-c); The ileal mucosa changes were observed by HE staining; The pH value of the colon feces was determined by pH meter; And the content of short-chain fatty acids (SCFA) in feces was determined by gas chromatography. Results: The results showed that Cannabis Fructus oil increased the ratio of bacillus, reduced the ratio of cocci and the cecal coefficient, decreased the pH value of the colon, significantly improved the colon pathological changes of the model animals with unbroken membrane skin, regular glands and rich cup cells and fluff rich, increased the content of SCFAs in the intestine of mice, and significantly increased (P < 0.01) the content of acetic acid, propionic acid, butyric acid, isobutyric acid, and significantly reduced (P < 0.01) the content of isovaleric acid. Conclusion: It could conclude that Cannabis Fructus oil can up-regulate the ratio of D-galactose-induced mice intestinal bacteria structure, improve the intestinal microecology, so as to provide theoretical support for clinical application and product development of Cannabis Fructus oil.