Chemical constituent analysis of Gegen Qinlian Decoction based on UPLC-LTQ-Orbitrap-MS / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 1498-1507, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-846521
ABSTRACT
Objective:
Identification of chemical constituents from Gengen Qinlian Decoction by UPLC-LTQ-Orbitrap-MS.Methods:
The analysis was performed on Dikma Endeavorsil C18 (100 mm × 2.1 mm, 1.8 μm) with mobile phase of 0.1% formic acid water solution (A)-acetonitrile (B) for gradient elution at a flow rate of 0.3 mL/min. The UPLC-LTQ-Orbitrap-MS was equipped with an Electrospray ionization ion probe and MS1 and MS2 data of samples were collected in positive and negative ion mode, respectively.Results:
A total of 67 constituents were identified from Gegen Qinlian Decoction by reference substance identification, software prediction analysis and related literature reports, including 36 flavonoids, 12 alkaloids, four triterpenoids and triterpenoid saponins, and 15 other ingredients.Conclusion:
In this study, UPLC-LTQ-Orbitrap-MS was used to systematically elucidate the chemical constituents of Gegen Qinlian Decoction, and the fragmentation characteristics of its main chemical constituents were preliminarily explained and summarized, which provided a reference for the quality control and mechanism research of Gegen Qinlian Decoction.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2020
Tipo del documento:
Artículo
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