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Inhibitory effect of capsaicin on B16-F10 melanoma cell migration via the phosphatidylinositol 3-kinase/Akt/Rac1 signal pathway
Experimental & Molecular Medicine ; : 486-494, 2008.
Artículo en Inglés | WPRIM | ID: wpr-84654
ABSTRACT
Capsaicin (trans-8-methyl-N-vanillyl-6-nonenamide), the major pungent ingredient of red pepper, has been reported to possess anti-carcinogenic and anti-mutagenic activities. In this study, the anti-migration activity of capsaicin on highly metastatic B16-F10 melanoma cells was investigated. Capsaicin significantly inhibited the migration of melanoma cells without showing obvious cellular cytotoxicity at low doses. This effect correlated with the down-regulation of phosphatidylinositol 3-kinase (PI3-K) and its downstream target, Akt. Although B16-F10 cell migration was increased by the PI3-K activator through the activation of Akt, these PI3-K activator-induced phenomena were attenuated by capsaicin. Moreover, capsaicin was found to significantly inhibit Rac1 activity in a pull-down assay. These results demonstrate that capsaicin inhibits the migration of B16-F10 cells through the inhibition of the PI3-K/Akt/Rac1 signal pathway. The present investigation suggests that capsaicin targets PI3-K/Akt/ Rac1-mediated cellular events in B16-F10 melanoma cells. Consequently, capsaicin administration should be considered an effective approach for the suppression of invasion and metastasis in malignant melanoma chemotherapy.
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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Melanoma Experimental / Capsaicina / Immunoblotting / Transducción de Señal / Movimiento Celular / Supervivencia Celular / Proteína de Unión al GTP rac1 / Línea Celular Tumoral / Relación Dosis-Respuesta a Droga / Proteínas Proto-Oncogénicas c-akt Límite: Animales Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2008 Tipo del documento: Artículo

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Texto completo: Disponible Índice: WPRIM (Pacífico Occidental) Asunto principal: Melanoma Experimental / Capsaicina / Immunoblotting / Transducción de Señal / Movimiento Celular / Supervivencia Celular / Proteína de Unión al GTP rac1 / Línea Celular Tumoral / Relación Dosis-Respuesta a Droga / Proteínas Proto-Oncogénicas c-akt Límite: Animales Idioma: Inglés Revista: Experimental & Molecular Medicine Año: 2008 Tipo del documento: Artículo