Pharmacodynamic difference of Ribes diacanthum and Ribes mandshuricum on renal fibrosis / 中草药
Chinese Traditional and Herbal Drugs
;
(24): 127-134, 2020.
Artículo
en Chino
| WPRIM
| ID: wpr-846700
ABSTRACT
Objective:
To compare pharmacodynamic difference of Ribes diacanthum (RDP) and Ribes mandshuricum (RMK) treatment on renal fibrosis in vivo and in vitro.Methods:
Both of TGFβ1-induced HK-2 cell fibrosis model and UUO-induced kidney fibrosis mice model were used in the present study. The cell morphology, ratio of cell length to width, renal histopathology, protein expressions of α-SMA and E-cadherin in kidney tissues were evaluated through biological and pharmacological methods and technologies, including Western blot, immunohistochemistry, HE staining, Masson staining and so on. In addition, partial least squares-discriminant analysis (PLS-DA) was applied to analyze the renal histopathological score as well.Results:
RDP (1.5, 5, 15 μg/mL) and RMK (3, 10 μg/mL) effectively improved morphological changes and reduced the ratio of cell length to width in TGFβ1-induced HK-2 cell fibrosis; Moreover, RDP (40 mg/kg) and RMK (80 mg/kg) remarkably decreased the expression of α-SMA and increased the expression of E-cadherin in UUO mice model. The degree of pathological damage and fibrosis were also alleviated in both groups. PLS-DA analysis showed no significant difference in anti-fibrotic effects between RDP and RMK treatment.Conclusion:
Both RDP and RMK have anti-fibrosis effects on TGFβ1-induced HK-2 cell fibrosis model and UUO-induced kidney fibrosis mice model, and there is no significant difference between these two herbs.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Tipo de estudio:
Estudio pronóstico
Idioma:
Chino
Revista:
Chinese Traditional and Herbal Drugs
Año:
2020
Tipo del documento:
Artículo
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