Analysis of microbial community characteristics of Panax notoginseng based on high-throughput sequencing technology / 中草药

ABSTRACT

Objective: The purpose of this study was to obtain the bacterial communities structures of fresh and dry Panax notoginseng. Methods: The bacterial were sequenced by Illumina MiSeq high-throughput sequencing technology, and the biological information was analyzed. Results: A total of 637 OTUs were obtained, of which 15 were in common, 484 were from fresh P. notoginseng and 123 were from dry P. notoginseng respectively. The larger phylum of fresh sample was Proteobacteria (59.7%) and Firmicutes (40.1%) while the dry sample were Firmicutes (99.8%). The main genus of fresh sample was Enterobacter (37.4%) and Alkaliphilus (11.5%), while dry sample were Bacillus (54.6%) and Paenibaciiius (44.9%). The functional prediction of KEGG showed that the population of bacteria metabolizing terpenoids and polyketones of dry sample was higher than that of fresh sample. Conclusion: The diversity of microbiota in fresh P. notoginseng was higher than that of dry sample; The species with the metabolic function of interpenoids and polyketones of dry P. notoginseng was higher than that of fresh sample, which provides an important reference for screening the microorganism for biotransformation of saponins in P. notoginseng.