Quantitative analysis of renal blood flow perfusion using contrast-enhanced ultrasound in rats with hemorrhagic shock during resuscitation / 中国组织工程研究
Chinese Journal of Tissue Engineering Research
;
(53): 1201-1206, 2021.
Artículo
en Chino
| WPRIM
| ID: wpr-847136
ABSTRACT
BACKGROUND:
The amount of renal blood perfusion is often in parallel with renal function. If contrast-enhanced ultrasound (CEUS) can be used to quantitatively analyze changes in renal blood flow perfusion during resuscitation so as to indirectly reflect changes in renal function, this will provide a new way for the clinical assessment of hemorrhagic shock-reperfusion injury of the kidney.OBJECTIVE:
To establish a reperfusion model of severe hemorrhagic shock in Sprague-Dawly rats and to explore the value of CEUS parameters in evaluating renal perfusion.METHODS:
Twenty male Sprague-Dawley rats were randomly divided into four groups, five in each group sham operation group, and 2-, 6-, and 24-hour hemorrhagic shock reperfusion groups (2-, 6-, and 24-hour HS-R groups). CEUS examination was performed in each group, and the quantitative parameters of peak intensity, time to peak, area under curve, average transit time were checked and obtained. The correlation between time-intensity curve parameters and serum creatinine, urea nitrogen, renal tissue myeloperoxidase, endothelin 1, and prostaglandin E1 levels was analyzed. RESULTS ANDCONCLUSION:
Compared with the sham operation group, the pathological injury was significant in the HS-R groups, especially in the HS-R group, with increased peak intensity, time to peak, area under curve, and average transit time (P < 0.05) as well as increased serum creatinine and urea nitrogen levels. There were significant differences in peak arrival time and average transit time among groups. Bivariate Pearson’s correlation analysis showed that time to peak and average transit time were significantly correlated with serum creatinine, urea nitrogen, renal tissue myeloperoxidase, endothelin 1, and prostaglandin E1 levels. To conclude, (1) CEUS can effectively monitor and quantitatively evaluate the renal perfusion changes during the recovery of hemorrhagic shock. (2) Time to peak and average transit time are two indexes of the time-intensity curve with some reference value. (3) After hemorrhagic shock and reperfusion, the kidney injury is aggravated but not alleviated, and the injury is the most serious at 24 hours after resuscitation. (4) CEUS provides a new idea for clinical evaluation of hemorrhagic shock-reperfusion injury of the kidney.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Chinese Journal of Tissue Engineering Research
Año:
2021
Tipo del documento:
Artículo
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