Properties of a novel photocrosslinked fish collagen peptide-hyaluronic acid hydrogel / 中国组织工程研究

ABSTRACT

BACKGROUND: A novel photocrosslinked fish collagen peptide-hyaluronic acid hydrogel has been successfully prepared by the research group. OBJECTIVE: To investigate the microstructure and the swelling properties in vitro of the photocrosslinked fish collagen peptide-hyaluronic acid hydrogel, and its histocompatibility and degradability in vivo. METHODS: A new photocrosslinked fish collagen peptide-hyaluronic acid hydrogel was prepared by photocrosslinking, and then divided into groups A (30 g/L), B (50 g/L) and C (100 g/L) according to the content of fish collagen peptide. The microstructure of the hydrogel was observed by scanning electron microscope and the swelling properties of hydrogels were studied by swelling test in vitro. Fifteen male Sprague-Dawley rats (provided by Qingdao Qinda Breeding Co., Ltd.) were selected, and three kinds of photocrosslinked fish collagen peptide-hyaluronic acid hydrogels were implanted into the back of rats. At 1, 2, 4, 6 and 8 weeks after surgery, three rats were selected for detecting the histocompatibility and biodegradability of hydrogels. The study was approved by the Ethics Committee of School of Basic Medicine, Qingdao University. RESULTS AND CONCLUSION: (1) With the increase of fish collagen peptide content, the transparency of hydrogel decreased and the pore size of hydrogel decreased. (2) Swelling equilibrium was achieved within 100 minutes in each group, and the swelling velocity and equilibrium swelling rate were inversely proportional to the content of collagen peptide. The equilibrium swelling rate was highest in the group A, which was 1 582%. (3) The hydrogel had good histocompatibility, mild inflammatory reaction, no infection, hematoma or other complications in vivo. (4) At 1 week after implantation, inflammatory cell infiltration was observed surrounding the hydrogels. The level of inflammatory cells peaked at 2 weeks, and then decreased gradually. The count of inflammatory cells was highest in the group A, followed by group B, and lowest in the group C (P group B > group C (P < 0.05). (6) These results indicate that photocrosslinked fish collagen peptide-hyaluronic acid hydrogel has good histocompatibility and biodegradability in vivo, which can be adjusted by the content of fish collagen peptides.