Expression of linc01296 in hepatocellular carcinoma tissues and its role in proliferation, migration and invasion of hep3b cells / 肿瘤

ABSTRACT

Objective: To investigate the expression of long noncoding RNA (lncRNA) LINC01296 in hepatocellular carcinoma (HCC) tissues and cells, and to explore its effects on the proliferation, migration and invasion of Hep3B cells. Methods: The expression of LINC01296 in liver cancer cells, HCC tissues and para-cancerous tissues was analyzed using Gene Expression Profiling Interactive Analysis (GEPIA) database and real-time quantitative PCR. Moreover, the expression of LINC01296 in HCC cells and the normal hepatocyte cells was detected by real-time fluorescent quantitative PCR. After the lentivirus carrying LINC01296-overexpression or-silencing vector was infected into Hep3B cells, the cell proliferation, migration and invasion were detected by CCK-8 assay and Transwell chamber assay, respectively. The effect of LINC01296 on the expressions of epithelial-mesenchymal transition (EMT)-related proteins in Hep3B cells was detected by Western blotting. Results: The expression level of LINC01296 was significantly up-regulated in HCC tissues and cells (both P < 0.05). The overexpression of LINC01296 significantly promoted the proliferation, migration, invasion and EMT activities of Hep3B cells (all P < 0.01), whereas the results were opposite when LINC01296 was silenced (all P < 0.01). Conclusion: The expression level of LINC01296 is up-regulated in HCC tissues and cells, and it can promote the proliferation, migration and invasion of Hep3B cells by enhancing the activities of EMT.