Inhibitory effect of 2,3',4,5'-tetramethoxystilbene on growth of endometrial carcinoma xenografts / 肿瘤

ABSTRACT

Objective: To investigate the effect of 2,3',4,5'-tetramethoxystilbene (TMS) on the growth of xenograft tumors of endometrial carcinoma Ishikawa cells, to explore its molecular mechanism, and to evaluate the adverse reaction of TMS. Methods: Human xenograft models were established in nude mice by inoculation with human endometrial carcinoma Ishikawa cells, and randomly divided into TMS group and control group by subcutaneously injection with 10 mgkg-1d-1 TMS and equal volume of olive oil, respectively. The volume and weight of xenograft tumors were calculated. The body weight of mice and the wet weight of tumor, heart, liver, spleen, lung, kidney, uterus and ovary in mice were measured. The solid organ damage was detected by HE staining. The expressions of cytochrome P4501B1 (CYP1B1), extracellular signal-regulated kinase (ERK) and phospho-ERK (p-ERK) proteins in xenograft tumors were detected by SP immunohistochemistry method. Results: After subcutaneous injection of TMS, the volume (P 0.05). No obvious lesion was found in various organs of nude mice in each group. The expressions of CYP1B1, ERK and p-ERK proteins were weakly positive in xenograft tumors. Conclusion: TMS can obviously inhibit the growth of human endometrial carcinoma xenografts in nude mice, and has no obvious toxic and side effects on tumor-bearing nude mice.