The expression of RPS15a in colorectal cancer tissues and the effects of silencing RPS15a on the proliferation and apoptosis of colon cancer RKO cells / 肿瘤

ABSTRACT

Objective: To investigate the role of ribosomal protein S15a (RPS15a)in colorectal cancer.Methods: The expressions of RPS15a in 120 specimens of colorectalcancer tissues and the para-cancerous tissues as well as 120specimens of colorectal adenoma tissues were detected byimmunohistochemistry. The proliferation, cell cycle and apoptosisof colon cancer RKO cells after infection with recombinant lentivirusRPS15a-siRNA were detected by Cellomics cell counting assay, colony formation assay and FCM, respectively.Results: The positive rate of RPS15a expression in colorectal cancer tissues was significantlyhigher than those in the colorectal adenoma tissues (86.7% vs 25.8%, P < 0.001) andthe para-cancerous tissues (86.7% vs 11.7%, P < 0.001). The expression of RPS15a wassignificantly correlated with the TNM stage (P = 0.033) and the tumor differentiation (P <0.001) of colorectal cancer. RPS 15a silencing significantly suppressed the proliferation (P <0.01) and colony formation (P < 0.01) of the RKO cells, induced apoptosis (P < 0.01), andarrested the cell cycle at G2/M phase (P < 0.01).Conclusion: The expression of RPS15a in colorectal cancer tissues is higher than those in thecolorectal adenoma tissues and the para-cancerous tissues. Down-regulation of RPS 15aexpression can repress the proliferation and induce the apoptosis of RKO cells.