The biological characteristics of adriamycin-resistant breast cancer cell line / 肿瘤

ABSTRACT

Objective: To investigate biological characteristics of adriamycin (ADR)-resistant breast cancer cell line. Methods: Using MCF-7 cells as parent cell line, an ADR-resistant cell line named MCF-7/ADR was established. The half maximal inhibitory concentration (IC50) values of MCF-7/ADR and MCF-7 cells were detected by resazurin test. The ability of colony formation of MCF-7/ADR and MCF-7 cells was measured by colony formation assay. The percentages of CD44+/CD24- and acetaldehyde dehydrogenase 1 (ALDH1)+ subtypes of cancer stem cells in MCF-7/ADR and MCF-7 cells were detected by FCM and immunofluorescent assay, respectively. The expressions of ALDH1 and smoothened (Smo) and Gli1 proteins in Hedgehog signaling pathway related to modulation of stem cells in MCF-7/ADR and MCF-7 cells were detected by Western blotting. Results: The IC50 value of ADR in MCF-7/ADR cells was higher than that in MCF-7 cells (P < 0.01), the resistant fold was 385. The colony formation rate of MCF-7/ADR was higher than that of MCF-7 cells (P < 0.01). The percentages of CD44+/CD24- and ALDH1+ subtypes of cancer stem cells in MCF-7/ADR cells were higher than those in MCF-7 cells (both P<0.01). The expression levels of ALDH1 protein and the Smo and Gli1 proteins in Hedgehog pathway related to modulation of stem cells in MCF-7/ADR cells were higher than those in MCF-7 cells (P < 0.01, P<0.01 and P<0.05, respectively). Conclusion: The percentages of CD44+/CD24- and ALDH1+ subtypes of cancer stem cells in MCF-7/ADR cells are higher, and the expressions of ALDH1 protein and the Smo and Gli1 proteins in Hedgehog pathway related to modulation of stem cells in MCF-7/ADR cells are higher. This mechanism may be responsible for ADR resistance in breast cancer cells.