Effect of tissue factor on the biological behavior of gastric cancer cells / 肿瘤

ABSTRACT

Objective: To investigate the effect of tissue factors (TF) on the biological behaviors of human gastric cancer SGC7901 and MKN45 cells. Methods: The recombinant TF antisense and sense vectors (pcDNA3.1-antisense TF and pcDNA3.1-sense TF) were constructed and transfected into SGC7901 cells (high expression of TF) and MKN45 cells (no expression of TF), respectively. The cell proliferation, cell cycle distribution and invasion capability were detected by MTT method, flow cytometry (FCM) and Transwell method, respectively. Results: The expression level of TF protein in SGC7901 cells transfected with recombinant TF antisense vector was down-regulated. As compared with the parental cells and the cells transfected with an empty vector, the proliferation of SGC7901 cells transfected with recombinant TF antisense vector was obviously decreased (P < 0.05), the proliferation index (PI) dropped from 0.357 to 0.286, and the number of the cells moved through the artifical membrane was reduced (P < 0.01). The proliferation of MKN45 cells transfected with recombinant TF sense vector was obviously increased as compared with those of the parental cells and the cells transfected with an empty vector (P < 0.05), the PI was increased from 0.277 to 0.366, and the invasion ability was increased (P < 0.01). Conclusion: TF may be an important factor associated with proliferation, movement and invasion of gastric cancer cells.