The effects of NHE -1 on the proliferative and invasive abilities of esophageal carcinoma cell line KYSE-70 in vitro / 肿瘤

ABSTRACT

Objective: To observe the effects of NHE -1 (Na+/H+ exchanger 1) gene on the proliferative and invasive abilities of esophageal cancer cell line KYSE-70. Methods: The expressions of NHE-1 mRNA and protein in esophageal cancer EC-9706, KYSE-450, EC-1 and KYSE-70 cells were detected by realtime fluorescence quantitative-PCR and Western blotting, respectively. Chemically synthesized siRNA (small interference RNA) targeting NHE -1 was transfected into KYSE-70 cells. The expression levels of NHE-1 mRNA and protein in esophageal cancer KYSE-70 cells 24, 48 and 72 h after transfection with NHE-1 siRNA were detected by real-time fluorescence quantitative-PCR and Western blotting, respectively. The proliferative and invasive abilities of KYSE-70 cells were detected by MTT method and Boyden chamber assay, respectively. Results: NHE-1 mRNA and protein were highly expressed in KYSE-70 cells. As compared with the negative control (transfected with nonsense siRNA) and blank control (KYSE-70 cells without transfection), the expression levels of NHE-1 mRNA and protein in KYSE-70 were significantly decreased after transfection with siRNA targeting NHE -1 (all P < 0.05). As compared with the negative control, blank control and the KYSE-70 cells transfected with NHE-1 siRNA for 24 h, the proliferative and invasive abilities of KYSE-70 were inhibited after transfection with NHE-1 siRNA for 48 and 72 h (all P < 0.05). Conclusion: Silencing the expression of NHE -1 gene can inhibit the proliferative and invasive abilities of esophageal cancer KYSE-70 cells in vitro . NHE -1 gene is expected to become an important target for gene therapy of esophageal cancer. Copyright © 2013 by TUMOR.