Expression of miR-451 and its correlation with drug resistance in breast cancer cells / 肿瘤
Tumor
;
(12): 982-986, 2012.
Artículo
en Chino
| WPRIM
| ID: wpr-849015
ABSTRACT
Objective:
To investigate the expression of miR-451 in breast cancer cells and its correlation with drug resistance to ADM (adriamycin).Methods:
Stem-loop real-time PCR was used to detect the expression of miR-451 (microRNA-451) in breast cancer MCF-7 cells and their ADM-resistant cells (MCF-7/ADM). The mimics of miR-451 and the negative control RNA (NC) were transfected into MCF-7/ADM cells by LipofectAMINE 2000. The expression levels of MDR1 (multi-drug resistance gene 1) mRNA and P-gp (P-glycoprotein) protein were examined using real-time fluorescence quantitative PCR and Western blotting, respectively. MTT was used to analyze the proliferation of MCF-7/ADM cells transfected with miR-451 mimics and treated with ADM at different concentrations.Results:
The expression level of miR-451 was lower in MCF-7/ADM cells than in MCF-7 cells (P < 0.05). The expressions of MDR1 mRNA and P-gp protein were decreased in MCF-7/ADM cells after transfection with miR-451 mimics as compared with those in the NC group (P < 0.05). The sensitivity of MCF-7/ADM cells transfected with miR-451 mimics and treated with ADM was enhanced and the IC50 (half inhibition concentration) value of ADM in miR-451 mimics transfection group was less than that of the NC group (P < 0.05).Conclusion:
The expression of miR-451 is down-regulated in breast cancer ADM-resistant cells, which may play a vital role in drug resistance by regulating the expression of MDR1/P-gp. Copyright © 2012 by TUMOR.
Texto completo:
Disponible
Índice:
WPRIM (Pacífico Occidental)
Idioma:
Chino
Revista:
Tumor
Año:
2012
Tipo del documento:
Artículo
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